Infection assays in Arabidopsis reveal candidate effectors from the poplar rust fungus that promote susceptibility to bacteria and oomycete pathogens.
Identifieur interne : 000018 ( Main/Exploration ); précédent : 000017; suivant : 000019Infection assays in Arabidopsis reveal candidate effectors from the poplar rust fungus that promote susceptibility to bacteria and oomycete pathogens.
Auteurs : Hugo Germain [Canada] ; David L. Joly ; Caroline Mireault [Canada] ; Mélodie B. Plourde [Canada] ; Claire Letanneur [Canada] ; Donald Stewart ; Marie-Josée Morency ; Benjamin Petre [Royaume-Uni, France] ; Sébastien Duplessis [France] ; Armand SéguinSource :
- Molecular plant pathology [ 1364-3703 ] ; 2018.
Descripteurs français
- KwdFr :
- Arabidopsis (microbiologie), Basidiomycota (pathogénicité), Chloroplastes (métabolisme), Cytosol (métabolisme), Dosage biologique (MeSH), Fractions subcellulaires (métabolisme), Immunité des plantes (MeSH), Maladies des plantes (microbiologie), Molécules contenant des motifs associés aux pathogènes (métabolisme), Oomycetes (croissance et développement), Oomycetes (pathogénicité), Plasmodesmes (métabolisme), Populus (microbiologie), Protéines fongiques (métabolisme), Pseudomonas syringae (croissance et développement), Pseudomonas syringae (pathogénicité), Végétaux génétiquement modifiés (MeSH).
- MESH :
- croissance et développement : Oomycetes, Pseudomonas syringae.
- microbiologie : Arabidopsis, Maladies des plantes, Populus.
- métabolisme : Chloroplastes, Cytosol, Fractions subcellulaires, Molécules contenant des motifs associés aux pathogènes, Plasmodesmes, Protéines fongiques.
- pathogénicité : Basidiomycota, Oomycetes, Pseudomonas syringae.
- Dosage biologique, Immunité des plantes, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Arabidopsis (microbiology), Basidiomycota (pathogenicity), Biological Assay (MeSH), Chloroplasts (metabolism), Cytosol (metabolism), Fungal Proteins (metabolism), Oomycetes (growth & development), Oomycetes (pathogenicity), Pathogen-Associated Molecular Pattern Molecules (metabolism), Plant Diseases (microbiology), Plant Immunity (MeSH), Plants, Genetically Modified (MeSH), Plasmodesmata (metabolism), Populus (microbiology), Pseudomonas syringae (growth & development), Pseudomonas syringae (pathogenicity), Subcellular Fractions (metabolism).
- MESH :
- chemical , metabolism : Fungal Proteins, Pathogen-Associated Molecular Pattern Molecules.
- growth & development : Oomycetes, Pseudomonas syringae.
- metabolism : Chloroplasts, Cytosol, Plasmodesmata, Subcellular Fractions.
- microbiology : Arabidopsis, Plant Diseases, Populus.
- pathogenicity : Basidiomycota, Oomycetes, Pseudomonas syringae.
- Biological Assay, Plant Immunity, Plants, Genetically Modified.
Abstract
Fungi of the Pucciniales order cause rust diseases which, altogether, affect thousands of plant species worldwide and pose a major threat to several crops. How rust effectors-virulence proteins delivered into infected tissues to modulate host functions-contribute to pathogen virulence remains poorly understood. Melampsora larici-populina is a devastating and widespread rust pathogen of poplar, and its genome encodes 1184 identified small secreted proteins that could potentially act as effectors. Here, following specific criteria, we selected 16 candidate effector proteins and characterized their virulence activities and subcellular localizations in the leaf cells of Arabidopsis thaliana. Infection assays using bacterial (Pseudomonas syringae) and oomycete (Hyaloperonospora arabidopsidis) pathogens revealed subsets of candidate effectors that enhanced or decreased pathogen leaf colonization. Confocal imaging of green fluorescent protein-tagged candidate effectors constitutively expressed in stable transgenic plants revealed that some protein fusions specifically accumulate in nuclei, chloroplasts, plasmodesmata and punctate cytosolic structures. Altogether, our analysis suggests that rust fungal candidate effectors target distinct cellular components in host cells to promote parasitic growth.
DOI: 10.1111/mpp.12514
PubMed: 27868319
PubMed Central: PMC6638046
Affiliations:
- Canada, France, Royaume-Uni
- Grand Est, Lorraine (région), Québec
- Trois-Rivières
- Université de Lorraine, Université du Québec à Trois-Rivières
Links toward previous steps (curation, corpus...)
Le document en format XML
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first="Hugo" last="Germain">Hugo Germain</name><affiliation wicri:level="4"><nlm:affiliation>Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, Trois-Rivières, QC, Canada, G9A 5H7.</nlm:affiliation><orgName type="university">Université du Québec à Trois-Rivières</orgName><country>Canada</country><placeName><settlement type="city">Trois-Rivières</settlement><region type="state">Québec</region></placeName></affiliation><affiliation><nlm:affiliation>Canadian Forest Service, Laurentian Forestry Centre, Natural Resources Canada, Sainte-Foy, QC, Canada, G1V 4C7.</nlm:affiliation><wicri:noCountry code="subField">G1V 4C7</wicri:noCountry></affiliation></author><author><name sortKey="Joly, David L" sort="Joly, David L" uniqKey="Joly D" first="David L" last="Joly">David L. 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type="state">Québec</region></placeName></affiliation></author><author><name sortKey="Stewart, Donald" sort="Stewart, Donald" uniqKey="Stewart D" first="Donald" last="Stewart">Donald Stewart</name><affiliation><nlm:affiliation>Canadian Forest Service, Laurentian Forestry Centre, Natural Resources Canada, Sainte-Foy, QC, Canada, G1V 4C7.</nlm:affiliation><wicri:noCountry code="subField">G1V 4C7</wicri:noCountry></affiliation></author><author><name sortKey="Morency, Marie Josee" sort="Morency, Marie Josee" uniqKey="Morency M" first="Marie-Josée" last="Morency">Marie-Josée Morency</name><affiliation><nlm:affiliation>Canadian Forest Service, Laurentian Forestry Centre, Natural Resources Canada, Sainte-Foy, QC, Canada, G1V 4C7.</nlm:affiliation><wicri:noCountry code="subField">G1V 4C7</wicri:noCountry></affiliation></author><author><name sortKey="Petre, Benjamin" sort="Petre, Benjamin" uniqKey="Petre B" first="Benjamin" last="Petre">Benjamin Petre</name><affiliation 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Sebastien" uniqKey="Duplessis S" first="Sébastien" last="Duplessis">Sébastien Duplessis</name><affiliation wicri:level="4"><nlm:affiliation>Centre INRA Nancy Lorraine, INRA, UMR 1136 Interactions Arbres/Microorganismes, INRA/Université de Lorraine, Champenoux, 54280, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre INRA Nancy Lorraine, INRA, UMR 1136 Interactions Arbres/Microorganismes, INRA/Université de Lorraine, Champenoux, 54280</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region></placeName><orgName type="university">Université de Lorraine</orgName></affiliation></author><author><name sortKey="Seguin, Armand" sort="Seguin, Armand" uniqKey="Seguin A" first="Armand" last="Séguin">Armand Séguin</name><affiliation><nlm:affiliation>Canadian Forest Service, Laurentian Forestry Centre, Natural Resources Canada, Sainte-Foy, QC, Canada, G1V 4C7.</nlm:affiliation><wicri:noCountry code="subField">G1V 4C7</wicri:noCountry></affiliation></author></analytic><series><title level="j">Molecular plant pathology</title><idno type="eISSN">1364-3703</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Arabidopsis (microbiology)</term><term>Basidiomycota (pathogenicity)</term><term>Biological Assay (MeSH)</term><term>Chloroplasts (metabolism)</term><term>Cytosol (metabolism)</term><term>Fungal Proteins (metabolism)</term><term>Oomycetes (growth & development)</term><term>Oomycetes (pathogenicity)</term><term>Pathogen-Associated Molecular Pattern Molecules (metabolism)</term><term>Plant Diseases (microbiology)</term><term>Plant Immunity (MeSH)</term><term>Plants, Genetically Modified (MeSH)</term><term>Plasmodesmata (metabolism)</term><term>Populus (microbiology)</term><term>Pseudomonas syringae (growth & development)</term><term>Pseudomonas syringae (pathogenicity)</term><term>Subcellular Fractions (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Arabidopsis (microbiologie)</term><term>Basidiomycota (pathogénicité)</term><term>Chloroplastes (métabolisme)</term><term>Cytosol (métabolisme)</term><term>Dosage biologique (MeSH)</term><term>Fractions subcellulaires (métabolisme)</term><term>Immunité des plantes (MeSH)</term><term>Maladies des plantes (microbiologie)</term><term>Molécules contenant des motifs associés aux pathogènes (métabolisme)</term><term>Oomycetes (croissance et développement)</term><term>Oomycetes (pathogénicité)</term><term>Plasmodesmes (métabolisme)</term><term>Populus (microbiologie)</term><term>Protéines fongiques (métabolisme)</term><term>Pseudomonas syringae (croissance et développement)</term><term>Pseudomonas syringae (pathogénicité)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Fungal Proteins</term><term>Pathogen-Associated Molecular Pattern Molecules</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Oomycetes</term><term>Pseudomonas syringae</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Oomycetes</term><term>Pseudomonas syringae</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Chloroplasts</term><term>Cytosol</term><term>Plasmodesmata</term><term>Subcellular Fractions</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Arabidopsis</term><term>Maladies des plantes</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Arabidopsis</term><term>Plant Diseases</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Chloroplastes</term><term>Cytosol</term><term>Fractions subcellulaires</term><term>Molécules contenant des motifs associés aux pathogènes</term><term>Plasmodesmes</term><term>Protéines fongiques</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Basidiomycota</term><term>Oomycetes</term><term>Pseudomonas syringae</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Basidiomycota</term><term>Oomycetes</term><term>Pseudomonas syringae</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biological Assay</term><term>Plant Immunity</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Dosage biologique</term><term>Immunité des plantes</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Fungi of the Pucciniales order cause rust diseases which, altogether, affect thousands of plant species worldwide and pose a major threat to several crops. How rust effectors-virulence proteins delivered into infected tissues to modulate host functions-contribute to pathogen virulence remains poorly understood. Melampsora larici-populina is a devastating and widespread rust pathogen of poplar, and its genome encodes 1184 identified small secreted proteins that could potentially act as effectors. Here, following specific criteria, we selected 16 candidate effector proteins and characterized their virulence activities and subcellular localizations in the leaf cells of Arabidopsis thaliana. Infection assays using bacterial (Pseudomonas syringae) and oomycete (Hyaloperonospora arabidopsidis) pathogens revealed subsets of candidate effectors that enhanced or decreased pathogen leaf colonization. Confocal imaging of green fluorescent protein-tagged candidate effectors constitutively expressed in stable transgenic plants revealed that some protein fusions specifically accumulate in nuclei, chloroplasts, plasmodesmata and punctate cytosolic structures. Altogether, our analysis suggests that rust fungal candidate effectors target distinct cellular components in host cells to promote parasitic growth.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">27868319</PMID><DateCompleted><Year>2018</Year><Month>07</Month><Day>23</Day></DateCompleted><DateRevised><Year>2020</Year><Month>02</Month><Day>25</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1364-3703</ISSN><JournalIssue CitedMedium="Internet"><Volume>19</Volume><Issue>1</Issue><PubDate><Year>2018</Year><Month>01</Month></PubDate></JournalIssue><Title>Molecular plant pathology</Title><ISOAbbreviation>Mol Plant Pathol</ISOAbbreviation></Journal><ArticleTitle>Infection assays in Arabidopsis reveal candidate effectors from the poplar rust fungus that promote susceptibility to bacteria and oomycete pathogens.</ArticleTitle><Pagination><MedlinePgn>191-200</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/mpp.12514</ELocationID><Abstract><AbstractText>Fungi of the Pucciniales order cause rust diseases which, altogether, affect thousands of plant species worldwide and pose a major threat to several crops. How rust effectors-virulence proteins delivered into infected tissues to modulate host functions-contribute to pathogen virulence remains poorly understood. Melampsora larici-populina is a devastating and widespread rust pathogen of poplar, and its genome encodes 1184 identified small secreted proteins that could potentially act as effectors. Here, following specific criteria, we selected 16 candidate effector proteins and characterized their virulence activities and subcellular localizations in the leaf cells of Arabidopsis thaliana. Infection assays using bacterial (Pseudomonas syringae) and oomycete (Hyaloperonospora arabidopsidis) pathogens revealed subsets of candidate effectors that enhanced or decreased pathogen leaf colonization. Confocal imaging of green fluorescent protein-tagged candidate effectors constitutively expressed in stable transgenic plants revealed that some protein fusions specifically accumulate in nuclei, chloroplasts, plasmodesmata and punctate cytosolic structures. Altogether, our analysis suggests that rust fungal candidate effectors target distinct cellular components in host cells to promote parasitic growth.</AbstractText><CopyrightInformation>© 2016 BSPP AND JOHN WILEY & SONS LTD.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Germain</LastName><ForeName>Hugo</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, Trois-Rivières, QC, Canada, G9A 5H7.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Canadian Forest Service, Laurentian Forestry Centre, Natural Resources Canada, Sainte-Foy, QC, Canada, G1V 4C7.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Joly</LastName><ForeName>David L</ForeName><Initials>DL</Initials><AffiliationInfo><Affiliation>Département de Biologie, Université de Moncton, Moncton, NB, Canada, E1A 3E9.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mireault</LastName><ForeName>Caroline</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, Trois-Rivières, QC, Canada, G9A 5H7.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Plourde</LastName><ForeName>Mélodie B</ForeName><Initials>MB</Initials><AffiliationInfo><Affiliation>Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, Trois-Rivières, QC, Canada, G9A 5H7.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Letanneur</LastName><ForeName>Claire</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Department of Chemistry, Biochemistry and Physics, Université du Québec à Trois-Rivières, Trois-Rivières, QC, Canada, G9A 5H7.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Stewart</LastName><ForeName>Donald</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Canadian Forest Service, Laurentian Forestry Centre, Natural Resources Canada, Sainte-Foy, QC, Canada, G1V 4C7.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Morency</LastName><ForeName>Marie-Josée</ForeName><Initials>MJ</Initials><AffiliationInfo><Affiliation>Canadian Forest Service, Laurentian Forestry Centre, Natural Resources Canada, Sainte-Foy, QC, Canada, G1V 4C7.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Petre</LastName><ForeName>Benjamin</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>The Sainsbury Laboratory, Norwich Research Park, Norwich, NR4 7UH, UK.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Centre INRA Nancy Lorraine, INRA, UMR 1136 Interactions Arbres/Microorganismes, INRA/Université de Lorraine, Champenoux, 54280, France.</Affiliation></AffiliationInfo></Author><Author 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Joly</name><name sortKey="Morency, Marie Josee" sort="Morency, Marie Josee" uniqKey="Morency M" first="Marie-Josée" last="Morency">Marie-Josée Morency</name><name sortKey="Seguin, Armand" sort="Seguin, Armand" uniqKey="Seguin A" first="Armand" last="Séguin">Armand Séguin</name><name sortKey="Stewart, Donald" sort="Stewart, Donald" uniqKey="Stewart D" first="Donald" last="Stewart">Donald Stewart</name></noCountry><country name="Canada"><region name="Québec"><name sortKey="Germain, Hugo" sort="Germain, Hugo" uniqKey="Germain H" first="Hugo" last="Germain">Hugo Germain</name></region><name sortKey="Letanneur, Claire" sort="Letanneur, Claire" uniqKey="Letanneur C" first="Claire" last="Letanneur">Claire Letanneur</name><name sortKey="Mireault, Caroline" sort="Mireault, Caroline" uniqKey="Mireault C" first="Caroline" last="Mireault">Caroline Mireault</name><name sortKey="Plourde, Melodie B" sort="Plourde, Melodie B" uniqKey="Plourde M" first="Mélodie B" last="Plourde">Mélodie B. Plourde</name></country><country name="Royaume-Uni"><noRegion><name sortKey="Petre, Benjamin" sort="Petre, Benjamin" uniqKey="Petre B" first="Benjamin" last="Petre">Benjamin Petre</name></noRegion></country><country name="France"><region name="Grand Est"><name sortKey="Petre, Benjamin" sort="Petre, Benjamin" uniqKey="Petre B" first="Benjamin" last="Petre">Benjamin Petre</name></region><name sortKey="Duplessis, Sebastien" sort="Duplessis, Sebastien" uniqKey="Duplessis S" first="Sébastien" last="Duplessis">Sébastien Duplessis</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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